How do our antibodies stack-up?

Side-by-side comparisons of Bio-Techne and Santa Cruz antibodies

SANTA CRUZ IS NO LONGER SELLING POLYCLONAL ANTIBODIES: As a result of a groundbreaking USDA ruling, a large number of Santa Cruz polyclonal antibodies were discontinued December 31, 2016.  In light of this news, Bio-Techne is working overtime to support your research and keep your experiments moving forward with minimal to no interruption.  

Get a match

THE RISK OF TESTING SANTA CRUZ’S TOP SELLING ANTIBODIES: As part of our internal effort to quell your concerns about switching antibody suppliers, we have begun putting our own antibodies to the test. We picked a handful of Santa Cruz’s most cited antibodies and performed head-to-head comparisons on multiple applications ranging from western blot to flow cytometry. Our decision to test Santa Cruz’s most cited antibodies was risky, but deliberate. We understood that their top selling antibodies were validated by thousands of peer-reviewed publications and heavily relied on by many labs. There was the obvious risk that our antibodies might not stack up against their best sellers.  

OUR COMMITMENT TO YOUR RESEARCH: However, at Bio-Techne, our commitment to your research compelled us to take that risk anyway. We want you to make the most informed decisions about your research, and we felt we could help by providing the data here for your personal review. As part of our commitment, Bio-Techne has started publishing the results of our side-by-side comparisons. The first batch of data can be found below, and we will continue to test our antibodies against equivalent Santa Cruz products.  

Side by side data comparison:

Target	Cat #	Reactivity	Applications	Pubs	WB	Flow	ICC	IHC
53BP1	SC-10911 NB100-304 MAB18772	Hu, Av, Eq, Bv Hu, Mu, Rt, Fi, Gt, Pm Hu	WB, IF, ELISA WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP WB, IHC, ICC	1 248
Actin	SC-1616 NBP2-25142 NB100-74340	Hu, Mo, Rt Hu, Mo, Rt Hu, Mo, Rt	WB, IP, IF, IHC, Flow WB, ICC, Flow WB, IP, IF, IHC, Flow	2598 0 14
Axl	SC-1096 AF154	Mu, Rt, Hu Hu	WB, IP, IF, IHC-P WB, Flow, IHC, Block, CyTOF-ready	20 19
beta-Actin	SC-47778 NBP1-47423 NB600-501 MAB8929	Hu, Mo, Rt Hu, Mo, Rt Hu, Mo, Rt Hu, Mo, Rt	WB, IF, IHC WB, Flow, ICC, IF WB, IP, IF, IHC, Flow WB	1940 0 240 1
Caspase-3	SC-7148 NB100-56708 NB100-56112 NB500-210 AF-605-NA	Mu, Rt, Hu, Eq, Ca, Po Hu, Mu, Rt, Po, ChHa, Ma Hu, Mu, Rt, Ca, Ge Hu, Mu, Rt Hu, Mu	WB, IP, IF, ELISA WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready WB, Simple Western, IHC, IHC-Fr, IHC-P, IP, ChIP WB, Simple Western, IHC, IHC-P WB, Simple Western, IP	521 101 11 17 5
CDK2	SC-163 NB100-2721 AF4654	Hu, Mo, Rt Hu, Mo Hu, Mo	WB, IF, IP WB, Flow WB, IHC, ICC	939 1 0
c-Fos	SC-52 AF7254	Mu, Rt, Hu, Eq, Fe Hu	WB, IP, IF, IHC-P, FCM, ELISA WB	599
EGFR	SC-03-G AF231	Mu, Rt, Hu, Eq, Ca, Bv, Po Hu	WB, IP, IF, IHC-P, ELISA WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap)	26 6
ErbB4	SC-283 AF1131 MAB1131	Mu, Rt, Hu, Av, Eq, Ca, Bv, Po Hu Hu	WB, IP, IF, IHC-P, FCM, ELISA WB, Flow, CyTOF-ready WB, IHC	154 1 3
ERK1	SC-94 AF1940 MAB1940	Mu, Rt, Hu, Ch, Fr, Eq, Ca, Bv, Po, Av Hu Hu	WB, IP, IF, IHC-P, FCM, ELISA WB WB, IHC, ICC	652
ERK2	SC-154 NB110-60506 AF1230 MAB1230	Hu, Mo, Rt Hu, Mo, Rt Hu, Mo, Rt Hu, Mo, Rt	WB, IP, IF, IHC, Flow WB, ICC, IHC, ELISA WB, IHC WB, IHC, ICC	1138 0 0 0
GAPDH	SC-25778 SC-47724 NB300-328 NBP1-47339 AF5718 MAB5718	Hu, Mo, Rt Hu, Mo, Rt Hu, Mo, Rt Hu, Mo Hu, Mo, Rt Hu, Mo, Rt	WB, IP, IF, IHC WB, IP, IF, IHC WB, ICC, IF, IHC WB, ICC, IF, IHC WB, IHC WB, IHC	1177 334 33 4 4 1
HIF-1 alpha	SC-10790 SC-13515 AF1935 MAB1536	Mu, Hu, Rt, Ca, Bv Mu, Rt, Hu, Bv, Po Hu, Mu, Rt Hu, Mu, Rt	WB, IP, IF, ELISA WB, IP, IF, IHC-P WB, SW, IP, ChIP WB, SW, IP	118 46 12 12
IL-1 beta	SC-1252 AF-201-NA AF-401-NA MAB601 MAB6964	Mu, Rt Hu Mu Hu Hu	WB, IP, IF, ELISA WB, ICC, Neut WB, Simple Western, IHC, ICC, Neut WB, ELISA(Cap), ICC, Neut WB, Simple Western, ICC	28 17 58 19 1
anti-goat IgG (H+L) [HRP]	SC-2020 NBP1-74822 HAF109 HAF017	Gt Gt Gt Gt	WB WB, IHC WB, WB	1066 0 13 11
anti-rabbit IgG (H+L) [HRP]	SC-2004 NBP2-30348H NBP1-75297 HAF008	Rb Rb Rb Rb	WB WB WB, IHC, Flow WB	1692 4 1 12
anti-mouse IgG (H+L) [HRP]	SC-2005 NBP1-75144 HAF007 HAF018	Mo Mo Mo Mo	WB WB, IHC WB WB	1471 0 16 2
HA-Tag	SC-805 SC-7392 NB600-362 NB600-363 MAB0601 MAB6875	Multi Multi Multi Multi Multi Multi	WB, IP, IF WB,IP, FC, ICC/IF WB, ICC/IF, IP WB, ICC/IF, ChIP, IHC, IP WB, IP, ICC WB, ICFlow	696 696 11 59   0
IkB-alpha	SC-371 NB100-56507 AF4299 MAB4299	Hu Hu, Mu, Rt Hu, Mu Hu	WB, Flow, ICC/IF, IHC, IP WB WB, IP, IF/ICC, IHC, Flow WB, IHC	139 32 0 1
LC3B	SC-28266 MAB85581 NBP2-46892 NB100-2220	Hu, Mu, Rt, Eq, Ca, Bv, Po, Av Hu, Mu, Rt, Po, Bt, Bv, Fi, Xp, Ze Hu, Mu, Rt, Po, Ba, Bv, Ca, Pm, SyHa, Ze	WB, IP, IF, ELISA WB, Simple Western WB, ICC/IF, IHC, IHC-P, IP WB, Simple Western, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA	34   2 551
Nanog	SC-33759 SC-293121 AF1997 AF2729	Hu Hu Hu Mu	WB, IP, IF, ELISA WB, IP, IF, ELISA WB, ChIP, ICC WB, CyTOF-ready, ICC, ICFlow	19 1 51 12
Oct-3/4	SC-8628 AF1759 MAB17591	Mu, Rt, Hu, Eq, Po Hu, Mu Hu	WB, IP, IF, ELISA WB, Simple Western, IP, ChIP, ICC WB, CyTOF-ready, ICC, ICFlow	76 14 1
p21	SC-397 NBP2-44525 AF1047	Hu, Mo, Rt Hu Hu	WB, IP, IF, IHC WB, Flow, IHC, IF WB, IP, IHC, ICC, Flow	1269 0 1
p53	SC-126 SC-6243 NBP2-29453 AF1355 MAB1355	Hu Hu, Mo Hu, Mk Hu, Mo, Rt Hu, Mo, Rt	WB, IP, IF, IHC, Flow WB, IP, IF, IHC WB, Flow, IHC, IF, IP WB, IP, ICC WB, IP, ICC	1584 901 2 4 3
pan Cytokeratin	SC-15367 NBP2-29429	Mu, Rt, Hu, Eq, Bv, Po Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb	WB, IP, IF, IHC-P, ELISA WB, Flow, ICC/IF, IHC-Fr, IHC-P	25 9
RelA/NFkB p65	SC-372 SC-109 NB100-56712 AF5078 MAB5078	Hu Hu Hu, Mu, Rt Hu, Mu Hu, Mu	WB, IP, IF/ICC,IHC, Flow ChIP, IP, WB, IHC, IF/ICC, Flow WB, Flow, IHC WB, IHC, ICC WB, ICC	139 390 9 1 0
SOX2	SC-17320 SC-365823 AF2018 MAB2018R	Mu, Rt, Hu, Av, Eq, Ca, Bv, Po, Av Mu, Rt, Hu, Eq, Ca, Bv Hu, Mu, Rt Hu, Mu, Rt	WB, IP, IF, IHC-P, ELISA WB, IP, IF, IHC-P, ELISA WB, Simple Western, ChIP, ICC WB, ICFlow	117 7 39
Survivin	SC-17779 NB500-201 MAB886 MAB8861 AF6471 AF886	Mu, Rt, Hu Hu, Mu, Rt, Ca, Fe, GP, Ha Hu Hu Hu Hu	WB, IP, IF, IHC-P, ELISA WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP CyTOF-ready, ICFlow WB, Simple Western, ICC WB, Simple Western, Flow, IHC, CyTOF-ready WB, Simple Western, IHC	139 277 1   2 32

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